TY  - INPR
T1  - In vitro evaluation of the capacity of a monofilament debrider* to remove biofilm and the efficacy of different wound dressings to prevent biofilm re-growth
T5  - Poster presented at EWMA 2014
LA  - English
AU  - Reddersen, Kirsten
AU  - Wiegand, Cornelia
AU  - Abel, Martin
AU  - Muldoon, Jeanette
AU  - Ruth, Peter
AU  - Hipler, Uta Christina
AB  - Aim: 
Biofilm development is a major impediment of wound healing. Current research targets antibiofilm strategies to restore optimal wound-healing. Combined treatment involving debridement and addition of antibacterial agents may provide high success rates. A monofilament debrider* consisting of polyester presents a fast and painless option for biofilm removal. We analyzed the re-growth properties of biofilm underneath different wound dressings.

Methods: 
A S.aureus biofilm was cultivated on glass plates. The monofilament debrider* was used to wipe the glass plates under standardized conditions (p=0.067N/cm2, v=1.6cm/s). Afterwards, glass plates were covered with various antimicrobially active wound dressings# and incubated for 24h at 37°C. Then, dressings were removed and glass plates further incubated for 48h. Biofilm on the glass plates was evaluated directly after dressing removal and following 48h re-growth period using the fluorescent alamar blue assay.

Results: 
It was shown that the monofilament debrider* effectively removed biofilm. It was observed that subsequent treatment with dressings reduced formation of new biomass. Significantly fewer bacteria were found after incubation with dressings containing antimicrobials. Polihexanide-containing dressings further exhibited a persistent decrease of biofilm re-growth, while biofilm quickly reformed in untreated controls and after removal of antimicrobial-free and silver-containing dressings.

Conclusions: 
It can be concluded that the combination of biofilm removal on the infected or critically colonized wound using a monofilament debrider* and subsequent treatment with antimicrobial dressings presents a successful antibiofilm strategy.
CY  - Madrid, Spain
Y2  - 1400018400
ER  -