TY  - INPR
T1  - DETERMINATION OF THE REDUCTION OF BIOFILM IN VITRO DURING WOUND CLEANSING USING A MONOFILAMENT DEBRIDER* AND CONVENTIONAL COTTON GAUZE
T5  - Poster presented at EWMA 2014
LA  - English
AU  - Wiegand, Cornelia
AU  - Reddersen, Kirsten
AU  - Abel, Martin
AU  - Muldoon, Jeanette
AU  - Ruth, Peter
AU  - Hipler, Uta Christina
AB  - Aim:
Debridement and removal of biofilm is a major challenge in treatment of patients with chronic wounds. Surgical debridement requires trained personal, operation theatre and is often associated with pain but conventional methods relaying on cotton gauze may not be enough. A monofilament debrider* consisting of polyester fibres presents a fast and almost painless option for debridement. Hence, we have investigated the capacity of this monofilament debrider* to remove biofilm in vitro and compared it to cotton gauze**.

Methods:
For the wound debridement model, a S.aureus biofilm is cultivated on glass plates. The monofilament debrider* and conventional cotton gauze** were used to debride/clean the glass plates under standardized conditions (p=0.067N/cm2, v=1.6cm/s). Afterwards, the glass plates were stained with crystal violet to visualize the bacteria residuals. Plate images were obtained and all images were processed using ImageJ 1.45m.

Results:
Monofilament debrider* and cotton gauze** initially exhibited a comparable cleansing performance. However, the monofilament debrider* demonstrated a significantly higher cleansing capacity. While the monofilament debrider’ was able to achieve a retained high reduction of the biofilm over wiping several plates, gauze** quickly lost its efficacy.

Conclusions: The reduction of biofilm achieved using the monofilament debrider* is significantly higher than that of cotton gauze**. Moreover, it presents a non-invasive and therefore almost painless alternative to other. Hence, this technique should provide a valuable tool in the treatment of patients with chronic wounds to improve the quality of life as well as to safe costs.
CY  - Madrid, Spain
Y2  - 1400018400
ER  -