TY  - INPR
T1  - Establishment of a moist environment by a new foam dressing (NFD)* beneficial for wound healing in vitro
T5  - Poster presented at EWMA 2020
LA  - English
AU  - Wiegand, Cornelia
AU  - Morgner, Bianka
AU  - Abel, Martin
AU  - Lobitz, Nicole
AU  - Roes, Claas
AU  - Hipler, Uta Christina
AB  - Aim: Foam dressings are thought to aid in the establishment of a beneficial moist wound environment as they exhibit excellent fluid management capacity by absorbing as well as donating fluid. Therefore, a scratch wound healing model using human fibroblasts and keratinocytes was developed depending on the donation of fluid by the applied dressings. The effect on cell proliferation and migration of NFD* and cotton gauze was investigated.

Methods: Human dermal fibroblasts (DF) and human epidermal keratinocytes (HaCaT) were seeded into 6-well-plates and cultivated for 48h before scratching. NFD and gauze were cut aseptically (d=3.5cm), soaked in medium and applied directly on the cell scratches without further medium addition. Cells were stained with hematoxylin/eosin after 1, 6, 24, and 48h. Scratches were photographed using a digital microscope and evaluated using the Image J program.

Results: It was found that NFD supported the scratch healing in vitro by promoting fibroblast and keratinocyte proliferation and migration through establishing a moist wound environment. Results were almost comparable to the medium control. In contrast, scratches covered with gauze remained open, probably due to the loss of moisture by drying of the wound dressing.

Conclusions: It could be shown that NFD is able to promote wound healing by effectively creating humid conditions favouring wound closure while gauze was not able to maintain the moist environment resulting in non-healing cell scratch wounds in vitro.

*NFD - Suprasorb® P sensitive (Lohmann&Rauscher)
CY  - Virtual Conference
Y2  - 1605654000
ER  -